X-ray data collection

X-ray data are collected using automated Rigaku X-ray equipment

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  • microfocus rotating anode X-ray generator (MicroMax-007HF)
  • confocal X-ray optics (Varimax-HF)
  • detector - an imaging plate (R-AXIS IV + +) and a CCD detector (Saturn - 944 +)
Synchrotron radiation is available upon request.


Number of data sets collected at this stage depends on applied phasing method:

  • Molecular Replacement (MR) method is used when the structure of homologous protein is available in the Protein Data Bank (PDB). Only one set of X-ray data is collected.
  • Multiple Isomorphous Replacement (MIR) method is used for the phase determination when homologous protein structure is not available. This method includes soaking of the crystals with heavy atoms for phase determination and more than one set of data is required.
  • Multiple Wavelength Anomalous Dispersion (MAD) method is used for the phase determination when homologous protein structure is not available. This method uses selenomethionine derivatives of the crystallized protein to determine the phases. In this case more than one set of data is needed.