Pharmacodynamics





The task of pharmacodynamics is to determine the drug mechanism of action at the molecular level and to analyze its physiological and biochemical effects on the body. Pharmacodynamic studies describes therefore the pharmacological effect of analyzed drug. Pharmacodynamics examines the processes such as binding of compounds by membrane receptors, regulation of enzyme activity in metabolic and regulatory pathways, signal transduction analysis by measurement of production of secondary messengers or protein phosphorylation, interaction of NCEs (New Chemical Entities) with cell membrane transporters, structural proteins as well as genetic material. Thus, pharmacodynamic studies enable determination of chemical structure of the drug with optimal Target Molecular Profile (TMP) and activity.

BioCentrum offers a wide range of pharmacodynamics studies that are commonly used during the lead optimization process. BioCentrum performs radioligand binding assays (tritium labeled), analysis of activation of signal transduction pathways, protein biomarkers expression analysis, characterization of protein-protein and protein-drug interactions, cytotoxicity tests or cell-based functional assays.