Initial crystallization screening

At first we try the pilot design of conditions from published crystallization conditions of the homologous protein (if these are publicly available). If those conditions will be unrepeatable, we can develop our, own, unique crystallization method using commercial and in-house buffers. At this stage typically over 1000 crystallization conditions are screened. Many crystallographic experiments are performed using commercial and in-house prepared buffers to establish the optimal conditions. The ability of the crystals to diffract the X-rays is determined followed by further optimization of the crystallization conditions to obtain individual crystals of high diffraction quality.

If the protein for crystallization is not prepared by BioCentrum, the Client is required to provide some additional information about macromolecule – please see section sample information .