Amino acid analysis

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Our laboratory offers amino acid analysis of proteins and peptides for determination of proteinaceus amino acids. Neither tryptophan nor cysteine is determined, no physiological nor modified amino acids are analyzed. The samples are hydrolyzed in gas-phase using 6M HCl during 24 h at 115 Celsius centigrades. Released amino acids are converted to phenylthiocarbamyl (PTC) derivatives and analysed on PicoTag 3.9x150 mm column (Waters) installed on a Waters HPLC unit. The detection limit is on a level of about 10 picomols but we suggest sending of a minimum 100 picomols of protein or peptide. We also accept proteins or peptides immobilized on a PVDF membranes. The samples for amino acid analysis should be prepared according to advices presented at sequencing of liquid samples section.   

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All queries concerning amino acid analysis, methodical questions as well as settlements concerning turnaround dates please direct to: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it . The shipping address for samples is: BioCentrum Sp. z o.o. ,Bobrzynskiego 14, 30-348 Krakow, Poland.

In publications please use the following description of the procedure applied: "Amino acid analysis was performed at BioCentrum Ltd. (Kraków, Poland). The protein samples were hydrolyzed in gas phase using 6M HCl at 115 deg. C for 24 h. The liberated amino acids were converted into phenylthiocarbamyl (PTC) derivatives and analyzed by high-pressure liquid chromatography (HPLC) on a PicoTag 3.9x150 mm column (Waters, Milford, MA, USA)."