Our services

• sequencing of isotope-labeled proteins and peptides. The manually collected hot amino acid residues can be counted in manual or automatic scintillation counter,

• determination of proteinaceous and peptide antigens using ELISA or Western blot techniques. There is possible colorimetric, fluorimetric as well as isotopic detection. We are able to analyze samples using both commercially available kits as well as to elaborate individual tests,

• determination of receptor-ligand interactions using different techniques (microplates, electrophoretic, isotopic), determination of binding curves,

• quantitative and qualitative comparative analyses of different proteinaceous and peptide compositions. The compared compound can be analyzed according to theis molecular masses, charge, amino acid composition and many other physicochemical parameter,

• elaboration, conduction and optimization of proteins or peptides purification procedures. There is possible to use ion-exchange, reversed-phase, hydrophobic and size-exclusion chromatography in low-, medium- and high-pressure modes,

• determination of LD50 or MIC doses for proteinaceous or peptide bactericidal agents. The assays can be performed using microdilution or colony counting methods. We are able also to determine the influence of physicochemical factors (ionic strength, pH, divalent cations) on the activity of analyzed samples using model systems (liposomes or transformed bacteria),
  

• enzymological determinations: enzyme-inhibitor interactions, determination of Kon using synthetic substrates, identification of proteinases specificity and other.